Objective: As a graduate student in Bioprocessing and Bioenergy at UIUC with hands-on experience in pilot-scale precision fermentation projects, I am passionate about advancing sustainable solutions through biomanufacturing
Desired Field: My interests lie in scaling microbial processes for production of enzymes, bio-based chemicals, and alternative proteins through data-driven biomanufacturing and pilot-scale optimization, thereby contributing to global Bioeconomy.
Projects: During my time in the FASTER program, I worked on scaling and optimizing microbial fermentations in pilot-scale bioreactors ranging from 16L to 1200L. My projects involved process development for biomass accumulation, metabolite production such as biopolymers, biofuels etc., including real-time monitoring of parameters such as pH, DO, and substrate utilization. I also contributed to media optimization and downstream processing tasks like centrifugation and chromatography, all while supporting cross-functional teams in troubleshooting and improving fermentation performance.
Equipment Capabilities:
Bench-top to pilot-scale bioreactors (16L, 260L, 400L, 1000L, 1200L)
HPLC (High-Performance Liquid Chromatography)
SDS-PAGE and Western Blotting systems
Centrifuges and lyophilizers
Ion exchange chromatography columns
YSI analyzers and spectrophotometers
Standard Processes:
Batch and fed-batch fermentation
Media preparation and sterilization (CIP/SIP)
Protein extraction and purification
Analytical feedstock preparation and analysis
Microbial culture handling and inoculum development
Bioprocess monitoring and control using BioCommand and PLC systems
Relevant Skills:
Bioreactor operation and scale-up (bench to pilot scale)
Fermentation (batch, fed-batch), media formulation, and sterilization (CIP/SIP)
Protein purification (centrifugation, ion exchange chromatography, lyophilization)
Analytical techniques (HPLC, SDS-PAGE, spectrophotometry, YSI analysis)
Bioprocess control systems (BioCommand, PLC)
Data analysis and documentation using Microsoft Excel and SuperPro Designer
Relevant Coursework:
Bioprocessing Biomass for Fuels
Advanced Bioprocess Coproducts
Plant Cell Bioprocessing
Bioreactors and Cell Culture Technologies
Project Management
Resume
Resume_Pulamolu R_2025_Revanth Pulamolu
